SOLUBILIZATION OF PARTICULATE PROTEINS AND NONELECTROLYTES BY CHAOTROPIC AGENTS
نویسندگان
چکیده
منابع مشابه
The dissociation of proteins by chaotropic salts.
Chaotropic salts were examined with a view to their use as protein-dissociating agents. The rank order of their ability to dissociate proteins followed the Hofmeister series for proteins which self-interact mainly via the formation of intermolecular hydrogen bonds (P-lactoglobulin A, hemoglobin). For hydrophobic associations @-casein, concanavalin A, chymotrypsin), misplacements in the Hofmeist...
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The effect of alkyltrimethylammonium ions on the thermostability of natural and modified DNA duplexes has been investigated. We have shown that the use of tetramethylammonium ions TMA+along with the chemical modification of duplexes allow the fine adjustment of T m and the possibility of obtaining several duplex systems with varied isostabilizedtemperatures, some of which show greater stability...
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Immune precipitates were solubilized by the alternative pathway of complement assembled from isolated proteins, i.e., C3, factor B, factor D, properdin, C3b inactivator (C3bINA), and beta 1H. The kinetic curves of solubilization in the isolated system and in EGTA-serum were virtually indistinguishable. No requirement of other factors was apparent. Deletion of C3bINA and beta 1H from the complet...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1969
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.62.4.1129